Publications (see Google scholar for full list and citations or my ORCHID ID)
2024 Yunhan Wu, Andrew Bell, Gavin H. Thomas, David N. Bolam, Frank Sargent, Nathalie Juge, Tracy Palmer and Emmanuele Severi (2024) Characterisation of anhydro-sialic acid transporters from mucosa-associated bacteria Microbiology doi.org/10.1099/mic.0.001448 Abigail J. Walklett, Emily K. P. Flack, Harriet S. Chidwick, Natasha E. Hatton, Tessa Keenan, Darshita Budhadev, Julia Walton, Gavin. H Thomas, Martin Anthony Fascione (2024) The Retaining Pse5Ac7Ac Pseudaminyltransferase KpsS1 Defines a Previously Unreported Glycosyltransferase Family (GT118). Angewandte Chem https://doi.org/10.1002/anie.202318523 Bryony K. Ackroyd, Eleanor J. Dodson, Javeria Mehboob, Adam A. Dowle, Gavin H. Thomas and Anthony J. Wilkinson (2024) Structure and ligand binding in the putative anti-microbial peptide transporter protein, YejA Microbiology doi.org/10.1099/mic.0.001430 Martin F. Peter, Jan A. Ruland, Yeojin Kim, Philipp Hendricks, Niels Schneberger, Jan Peter Siebrasse, Gavin H. Thomas, Ulrich Kubitscheck & Gregor Hagelueken. Conformational coupling of the sialic acid TRAP transporter HiSiaQM with its substrate binding protein HiSiaP (2024) Nature Comms volume 15, Article number: 217 (also BioRxiv. doi.org/10.1101/2023.03.04.531103) 2023 Rory Elston, Christopher Mulligan & GH Thomas. Flipping the switch: dynamic modulation of membrane transporter activity in bacteria. Microbiology 169 (11), 001412 mic.0.001412 James W Southwell, Keith S Wilson, Gavin H Thomas, Anne K Duhme-Klair. Augmentation of growth media for extreme iron-limitation in Escherichia coli. Access Microbiology, 000735. v1 acmi.0.000735.v1/ Konstantinos Drousiotis, Reyme Herman, Judith Hawkhead, Andrew Leech, Anthony Wilkinson, Gavin H Thomas (2023) Characterization of the l-arabinofuranose-specific GafABCD ABC transporter essential for l-arabinose-dependent growth of the lignocellulose-degrading bacterium Shewanella sp. ANA-3. Microbiology Mar;169(3). doi: 10.1099/mic.0.001308. CS Lau, A Dowle, GH Thomas, P Girr, LCM Mackinder (2023) A phase-separated CO2-fixing pyrenoid proteome determined by TurboID in Chlamydomonas reinhardtii. The Plant Cell, koad131 Michael Brockhurst, Jennifer Cavet, Stephen P. Diggle, David Grainger, Riccardo Mangenelli, Hana Sychrova, Isabel Martin-Verstraete, Martin Welch, Tracy Palmer and Gavin H. Thomas (2023) Shaping microbiology for 75 years: highlights of research published in Microbiology. Part 1 - Physiology and growth. Microbiology https://doi.org/10.1099/mic.0.001356 Michael Brockhurst, Jennifer Cavet, Stephen P. Diggle, David Grainger, Riccardo Mangenelli, Hana Sychrova, Isabel Martin-Verstraete, Martin Welch, Tracy Palmer and Gavin H. Thomas (2023) Shaping microbiology for 75 years: highlights of research published in Microbiology. Part 2 - Communities and evolution Microbiology https://doi.org/10.1099/mic.0.001357 James Southwell, Reyme Herman, Daniel Raines, Justin Clarke, Isabelle Boeswald, Thorsten Dreher, Sophie Gutenthaler, Nicole Schubert, Jana Seefeldt, Nils Metzler-Nolte, Gavin Thomas, Keith Wilson, Anne-Kathrin Duhme-Klair (2022) Siderophore‐linked Ruthenium Catalysts for Targeted Allyl Ester Prodrug Activation within Bacterial Cells. Chemistry–A European Journal 29:e202202536 Kahlan E Newman, Sarah N Tindall, Sophie L Mader, Syma Khalid, Gavin H Thomas & Marjan W Van Der Woude (2023) A novel fold for acyltransferase-3 (AT3) proteins provides a framework for transmembrane acyl-group transfer. eLife doi.org/10.7554/eLife.81547. With Digest Article "Moving the loop". 2022
Thomas, G.H. (2022) Microbial Musings - Winter 2022. Microbiology doi.org/10.1099/mic.0.001310 Hughes, AM, Darby DJ, Dodson EJ, Wilson SJ, Turkenburg, JP, Thomas GH and Wilkinson AJ (2022) Peptide transport in Bacillus subtilis–structure and specificity in the extracellular solute binding proteins OppA and DppE. Microbiology 168 (12), 001274 Thomas, G.H. (2022) Microbial Musings - Autumn 2022. Microbiology doi.org/10.1099/mic.0.001291 Lewis J. White, Matthew Rose, Michael Lawson, Domino Joyce, Alan M. Smith, Gavin H. Thomas , Kanchon K. Dasmahapatra, Mary E. Pownall (2022) Two closely related ureotelic fish species of the genus Alcolapia express different levels of ammonium transporters in gills. Biol Open bio.059575. https://doi.org/10.1242/bio.059575 Thomas, G.H. (2022) Microbial Musings - Summer 2022. Microbiology doi.org/10.1099/mic.0.001250 Martin F. Peter, Jan A. Ruland, Peer Depping, Niels Schneberger, Emmanuele Severi, Jonas Moecking, Karl Gatterdam, Sarah Tindall, Alexandre Durand, Veronika Heinz, Jan Peter Siebrasse, Paul-Albert Koenig, Matthias Geyer, Christine Ziegler, Ulrich Kubitscheck, Gavin H. Thomas & Gregor Hagelueken (2022) Structural and mechanistic analysis of a tripartite ATP-independent periplasmic TRAP transporter. Nature Comms 13, Article number: 4471. Martin F. Peter, Christian Gebhardt, Rebecca Mächtel, Gabriel G. Moya Muñoz, Janin Glaenzer, Alessandra Narducci, Gavin H. Thomas, Thorben Cordes & Gregor Hagelueken (2022) Cross-validation of distance measurements in proteins by PELDOR/DEER and single-molecule FRET. Nature Comms 13, Article number: 4396. Benjamin James Willson, Reyme Herman; Swen Langer; Gavin Hugh Thomas (2022) Improved furfural tolerance in Escherichia coli mediated by heterologous NADH-dependent benzyl alcohol dehydrogenases. Biochemical J 479 (10): 1045–1058. https://doi.org/10.1042/BCJ20210811 Thomas, G.H. (2022) Microbial Musings - Spring 2022. Microbiology doi.org/10.1099/mic.0.001205 Conor M. Black, Adrian J. Chu, Gavin H. Thomas, Anne Routledge, Anne- Kathrin Duhme-Klair (2022) Synthesis and antimicrobial activity of an SO2-releasing siderophore conjugate. J Inorganic Biochemistry. .234, September 2022, 111875. doi.org/10.1016/j.jinorgbio.2022.111875. Joseph P. Webb, Ana Carolina Paiva, Luca Rossoni, Amias Alstrom-Moore, Vicki Springthorpe, Sophi Vaude, Vivien Yeh, David-Paul Minde, Sven Langer, Heather Walker, Andrea Hounslow, David R. Nielsen, Tony Larson, Kathryn Lilley, Gill Stephens, Gavin H. Thomas, Boyan B. Bonev, David J. Kelly, Alex Conradie, Jeffrey Green (2022) Multi-omic based production strain improvement (MOBpsi) for bio-manufacturing of toxic chemicals. Metabolic Engineering, https://doi.org/10.1016/j.ymben.2022.03.004. Caroline Pearson, Sarah Tindall, Jennifer R. Potts, Gavin H. Thomas, Marjan W. van der Woude (2022) Diverse functions for acyltransferase-3 proteins in the modification of bacterial cell surfaces. Microbiology 168:3 https://doi.org/10.1099/mic.0.001146 Lucija Slemc, Jernej Jakše, Alessandro Filisetti, Damir Baranasic, Antonio Rodríguez-García, Francesco Del Carratore, Stefano Maria Marino, Jurica Zucko, Antonio Starcevic, Martin Šala, Mercedes Pérez-Bonilla,hMarinaSánchez-Hidalgo, Ignacio González, Fernando Reyes, Olga Genilloud. Vicki Springthorpe, Dušan Goranovic, Gregor Kosec, Gavin H. Thomas, Davide De Lucrezia, Hrvoje Petkovic . Miha Tome (2022) Reference-Grade Genome and Large Linear Plasmid of Streptomyces rimosus: Pushing the Limits of Nanopore Sequencing. Microbiology Spectrum 10.1128/spectrum.02434-21 Bisson, C., Salmon, R.C., West, L., Rafferty, J.B., Hitchcock, A., Thomas, G.H & David J. Kelly (2022) The structural basis for high-affinity uptake of lignin-derived aromatic compounds by proteobacterial TRAP transporters. The FEBS Journal. Vol 289, Issue2. Jan 2022 Pages 436-456 https://doi.org/10.1111/febs.16156 Lisa M. Miller, Matthew D. Simmons, Callum D. Silver, Thomas F. Krauss, Gavin H. Thomas, Steven D. Johnson and Anne-Kathrin Duhme-Klair (2022) 4, 573-581 Antibiotic-functionalized Gold Nanoparticles for the Detection of Active beta-lactamases. Nanoscale Advances DOI: 10.1039/D1NA00635E 2021 Severi, E., Rudden, M., Bell, A., Palmer, T., Juge, N and Gavin H. Thomas (2021) Multiple evolutionary origins reflect the importance of sialic acid transporters in the colonisation potential of bacterial pathogens and commensals. Microbial Genomics https://doi.org/10.1099/mgen.0.000614 Carratore. F.D, Iorio, M., Pérez-Bonilla, M., Schmidt, K., Pérez-Redondo, R., Sosio, M., Macdonald, S.J., Gyulev, I.S., Tsigkinopouloua, A., Thomas, G.H., Genilloud, O., Rodríguez-García A., Donadio, S., Breitling, R., and Eriko Takano (2021) Multi-omics Study of Planobispora rosea, Producer of the Thiopeptide Antibiotic GE2270A. mSystems. https://doi.org/10.1128/mSystems.00341-21 Meng, F., Dornau, A., McQueen-Mason, S.J., Thomas, G.H., Conradie, A & Jon McKechnie (2021) Bioethanol from autoclaved municipal solid waste: assessment of environmental and financial viability under policy contexts. Applied Energy 298:117118. doi.org/10.1016/j.apenergy.2021.117118 Breitling, R.; Avbelj, M.; Bilyk, O.; Del Carratore, F.; Filisetti, A.; Hanko , E.; Iorio , M.; Pérez Redondo, R.; Reyes, F.; Rudden, M.; Severi, E.; Slemc, L.; Schmidt, K.; Whittall, D.; Donadio, S.; Rodriguez Garcia , A.; Genilloud, O.; Kosec, G.; De Lucrezia , D. Petković, H.; Thomas, G.H. & Eriko Takano (2021) Synthetic biology approaches to actinomycete strain improvement. FEMS Microbiology Letters fnab060. Chidwick, H., Flack, E., Keenan, T., Walton, J., Thomas, G.H & Fascione M.A. (2021) Reconstitution and optimisation of the biosynthesis of bacterial sugar pseudaminic acid (Pse5Ac7Ac) enables preparative enzymatic synthesis of CMP-Pse5Ac7Ac. Scientific Reports 11, Article number: 4756. Peter, M.F., Christian Gebhard, Janin Glaenzer, Niels Schneberger, Marijn de Boer, Gavin H. Thomas, Thorben Cordes and Gregor Hagelueken (2021) Triggering closure of a sialic acid TRAP transporter substrate binding protein through binding of natural or artificial substrates. J. Mol. Biol 433(3):166756. doi: 10.1016/j.jmb.2020.166756. With Cover image (right). 2020 Lisa M. Miller; Reyme Herman; Ivan Gyulev; Thomas F. Krauss; Gavin H. Thomas and Anne-Kathrin Duhme-Klair (2020) Synthesis and biochemical evaluation of cephalosporin analogues equipped with chemical tethers. RSC Adv., 2020, 10, 36485-36494. DOI: 10.1039/D0RA04893C Herman, R; Bennett-Ness, C; Maqbool, A; Afzal, A; Leech, A & Gavin H. Thomas (2020) The Salmonella enterica serovar Typhimurium virulence factor STM3169 is a hexuronic acid binding protein component of a TRAP transporter. Microbiology doi.org/10.1099/mic.0.000967. Bell, A., Severi, E., Lee, M.O., Monaco, S., Latousakis, D., Angulo, J., Thomas, G.H., Naismith, J & Juge, N (2020) Uncovering a novel molecular mechanism for scavenging sialic acids in bacteria. J Biol Chem doi: 10.1074/jbc.RA120.014454. Sanderson, T; Black, C; Southwell, J; Wilde, E; Pandey, A; Herman, R; Thomas, G; Boros, E; Duhme-Klair, A; Routledge, A (2020) A Salmochelin S4-inspired Ciprofloxacin Trojan Horse Conjugate. ACS Infectious Diseases doi.org/10.1021/acsinfecdis.0c00568 Flack, E; Chidwick, H; Guchhait, G; Keenan, T; Budhadev, D; Huang, K; Both, P; Mas P; Ledru, H; Rui, S; Stafford, G; Shaw, J; Galan, M.C; Flitsch, S; Thomas, G; Fascione, M (2020) Biocatalytic Transfer of Pseudaminic Acid (Pse5Ac7Ac) using Promiscuous Sialyltransferases in a Chemoenzymatic Approach to Pse5Ac7Ac Containing Glycosides. ACS Catalysis 2020, 10, XXX, 9986–9993 Pearson, C.*, Tindall, S*., Herman, R., Jenkins, H. T., Bateman, A., Thomas, G. H., Potts, J. R. & Van Der Woude, M., (2020) Acetylation of surface carbohydrates in bacterial pathogens requires coordinated action of a two-domain membrane-bound acyltransferase. mBio DOI: 10.1128/mBio.01364-20 Rudden, M. M.*, Herman, R.*, Rose, M., Bawdon, D., Cox, D. S., Dodson, E., Holden, M. T. G., Wilkinson, A. J., James, A. G. & Thomas, G. H., (2020) The molecular basis of thioalcohol production in human body odour. Scientific Reports 10, Article number: 12500 Hall, R. J., Thorpe, S., Thomas, G. H. & Wood, A. J. (2020) Simulating the evolutionary trajectories of metabolic pathways for insect symbionts in the Sodalis genus. Microbial Genomics doi: 10.1099/mgen.0.000378 Dornau, D., Robson, J.F., Thomas, G.H. & Simon J. McQueen-Mason (2020) Robust microorganisms for biofuel and chemical production from municipal solid waste. Microbial Cell Factories 19, Article number: 68 Flack, E.P.K., Chidwick H.S., Best, M., Thomas G & Fascione, M.A. (2020) Synthetic approaches for accessing pseudaminic acid (Pse) bacterial glycans. ChemBioChem. doi:10.1002/cbic.202000019 2019 Bell, A., Brunt, J., Crost, E., Vaux, L., Nepravishta, R., Owen, C.D., Latousakis, L., , Xiao, A., Li, W., Chen, A., Walsh, M.A., Claesen, J., Angulo, J., Thomas, G.H. & Juge, N (2019) Elucidation of a sialic acid metabolism pathway in mucus-foraging Ruminococcus gnavus unravels mechanisms of bacterial adaptation to the gut. Nature Microbiology doi: 10.1038/s41564-019-0590-7 Willson, BJ., Chapman, L.N.M & THomas GH (2019) Evolutionary dynamics of membrane transporters and channels: enhancing function through fusion. Current Opinion in Genetics and Development. 58-59, October 2019, pages 76-86. Darby JF, Hopkins AP, Shimizu S, Roberts SM, Brannigan JA, Turkenburg JP, Thomas GH, Hubbard RE, Fischer M. (2019) Water networks can determine the affinity of ligand binding to proteins. J Am Chem Soc. 2019 Sep 13. doi: 10.1021/jacs.9b06275. [Epub ahead of print] Miller LM, Silver CD, Herman R, Duhme-Klair AK, Thomas GH, Krauss TF, Johnson SD (2019) Surface-Bound Antibiotic for the Detection of β-Lactamases. ACS Appl Mater Interfaces. 2019 Sep 11;11(36):32599-32604. doi: 10.1021/acsami.9b05793. Epub 2019 Aug 26. Joseph Webb, Vicki Springthorpe, Luca Rossoni, David-Paul Minde, Swen Langer, Heather Walker, Amias Alstrom-Moore, Tony Larson, Kathryn Lilley, Graham Eastham, Gill Stephens, Gavin H. Thomas, David J. Kelly, Jeffrey Green (2019) Systems Analyses Reveal the Resilience of Escherichia coli Physiology during Accumulation and Export of the Nonnative Organic Acid Citramalate. mSphere DOI: 10.1128/mSystems.00187-19 Bell, A., Severi E., Juge, N. and Thomas GH (2019) A special sugar:how sialic acid impacts on metabolism, health and disease. Microbiology Today May 62-65. Severi E & Thomas GH (2019) Antibiotic export: transporters involved in the final step of natural product production. Microbiology. Apr 9. doi: 10.1099/mic.0.000794. Ben J. Willson, Lindsey Dalzell, Liam N.M. Chapman, Gavin H. Thomas (2019) Enhanced functionalisation of major facilitator superfamily transporters via fusion of C-terminal protein domains is both extensive and varied in bacteria. Microbiology doi: 10.1099/mic.0.000771 Rebecca J. Hall, Lindsey A. Flanagan, Michael J. Bottery, Vicki Springthorpe, Stephen Thorpe, Alistair C. Darby, A. Jamie Wood, Gavin H. Thomas (2019) A Tale of Three Species: Adaptation of Sodalis glossinidius to Tsetse Biology, Wigglesworthia Metabolism, and Host Diet. mBio 10.1128/mBio.02106-18 Rayner M. L. Queiroz, Tom Smith, Eneko Villanueva, Maria Marti-Solano, Mie Monti, Mariavittoria Pizzinga, Dan-Mircea Mirea, Manasa Ramakrishna, Robert F. Harvey, Veronica Dezi, Gavin H. Thomas, Anne E. Willis & Kathryn S. Lilley (2019) Comprehensive identification of RNA–protein interactions in any organism using orthogonal organic phase separation (OOPS) Nature Biotech doi: 10.1038/s41587-018-0001-2 2018 Thomas GH (2018) Frederick Griffith (1877-1944) Oxford Dictionary of National Biography. Published 12th July 2018. Minhas GS, Bawdon D, Herman R, Rudden M, Stone AP, James AG, Thomas GH, Newstead S (2018) Structural basis of malodour precursor transport in the human axilla. Elife. 2018 Jul 3;7. pii: e34995. doi: 10.7554/eLife.34995 Vanderhoven J, Green J, Thomas GH. (2018) CBMNet: the 'Crossing Biological Membranes' network in industrial biotechnology and bioenergy. Biochem Soc Trans. 2018 Jul 19. pii: BST20170532. doi: 10.1042/BST20170532. Rosa, L.T., Springthorpe, V., Bianconi, M.E., Thomas, G.H. & Kelly D.J. (2018) Massive over-representation of solute-binding proteins (SBPs) from the tripartite tricarboxylate transporter (TTT) family in the genome of the a-proteobacterium Rhodoplanes sp. Z2-YC6860. Microbial Genomics April 18. DOI 10.1099/mgen.0.000176 Rosa LT, Bianconi ME, Thomas GH & Kelly DJ (2018) Tripartite ATP-independent periplasmic (TRAP) transporters & tripartite tricarboxylate transporters (TTT): From uptake to pathogenicity. Front. Cell. Infect. Microbiol. | doi: 10.3389/fcimb.2018.00033 Gyulev IS, Willson BJ, Hennessy RC, Krabben P, Jenkinson ER, Thomas GH (2018) Part by Part: Synthetic Biology Parts Used in Solventogenic Clostridia. ACS Synth Biol. 2018 Feb 12 8:33 doi: 10.1021/acssynbio.7b00327 2017 Thomas, G.H. (2017) On the pull: periplasmic trapping of sugars before transport. Mol Microbiol Apr 13. doi: 10.1111/mmi.13691 Glaenzer J, Peter MF, Thomas GH, Hagelueken G (2017) PELDOR Spectroscopy Reveals Two Defined States of a Sialic Acid TRAP Transporter SBP in Solution. Biophys J. 112(1):109-120. With associated Commentary: The Making and Breaking of a Substrate Trap by Gunner Jeschke. 2016 Thomas, G.H. (2016) Sialic acid acquisition in bacteria - one substrate, many transporters. Biochemical Society Transactions 44 (3) 760-765; DOI: 10.1042/BST20160056 2015 Ziesemer KA, Mann AE, Sankaranarayanan K, Schroeder H, Ozga AT, Brandt BW, Zaura E, Waters-Rist A, Hoogland M, Salazar-García DC, Aldenderfer M, Speller C, Hendy J, Weston D, MacDonald SJ, Thomas GH, Collins MJ, Lewis CM, Hofman C, Warinner C. (2015) Intrinsic challenges in ancient microbiome reconstruction using 16S rRNA gene amplification. Sci Rep. 5:16498. doi: 10.1038/srep16498. Maqbool, A, Horler, R.S.P., Muller, A., Wilkinson, A.J., Wilson K.S., & Thomas GH (2015) The substrate-binding protein in bacterial ABC transporters: dissecting roles in the evolution of substrate specificity. Biochemical Society Transactions 43 (5) 1011-1017; DOI: 10.1042/BST20150135 Fischer M, Hopkins AP, Severi E, Hawkhead J, Bawdon D, Watts AG, Hubbard RE, Thomas GH (2015) Tripartite ATP-independent periplasmic (TRAP) transporters use an arginine-mediated selectivity filter for high affinity substrate binding. J Biol Chem. Sep 5. pii: jbc.M115.656603 Bawdon, D; Cox, D.S.; Ashford, D.; James, A.G. & Thomas G.H. (2015) Identification of axillary Staphylococcus sp. involved in the production of the malodorous thioalcohol 3-methyl-3-sufanylhexan-1-ol . FEMS Microbiology Letters doi: 10.1093/femsle/fnv111 2014 International Glossina Genome Initiative (2014) Genome sequence of the tsetse fly (Glossina morsitans): vector of African trypanosomiasis. Science. Apr 25;344(6182):380-6 Atkin KE, Macdonald SJ, Brentnall AS, Potts JR, Thomas GH. (2014) A different path: revealing the function of staphylococcal proteins in biofilm formation. FEBS Lett. in press. [Epub ahead of print] Milner, S.J., Snelling, A.M., Kerr, K.G., Abd-El-Aziz, A., Thomas, G.H., Hubbard, R.E., Routledge, A., Duhme-Klair, A.K. (2014) Probing linker design in citric acid-ciprofloxacin conjugates. Bioinorganic & Medicinal Chemistry. 22:4499-4505. Milner, S.J., Carrick, C.T., Kerr, K.G., Snelling, A.M., Thomas, G.H., Dukme-Klair, A-K., & Routledge, A. (2014) Probing Bacterial Uptake of Glycosylated Ciprofloxacin Conjugates. ChemBioChem 15:466-471. Thomas, GH (2014) Frederick William Twort: not just bacteriophage. Microbiology Today 14:70-73. 2013 Macdonald, S.J., Thomas, G.H. & Douglas, A.E. (2013) Waste not, want not - nitrogen recycling by metabolic pathways shared between an animal and its symbiotic bacteria. The Biochemist. 35 (No. 4, August issue) Hopkins, A.P., Hawkhead, J.A., & Thomas, G.H. (2013) Transport and catabolism of the sialic acids N-glycolylneuraminic acid (Neu5Gc) and 3-keto-3-deoxy-D-glycero-D-galactonononic acid (KDN) by Escherichia coli K-12. FEMS Microbiol Lett. 347(1):14-22. Milner, S.J., Seve, A., Snelling, A.M., Thomas, G.H., Kerr, K.G., Routledge, A & Duhme-Klair A-K. (2013) Staphyloferrin A as siderophore-component in fluoroquinolone-based Trojan horse antibiotics. Organic & Biomolecular Chemistry. 11(21):3461-8. Basavanna, S., Chimalapati, S., Maqbool, A., Rubbo, B., Yuste, J., Wilson, R.J., Hosie, A., Ogunniyi, A.D., Paton, J.C., Thomas, G.H., and Brown, J.S. (2012) The effects of methionine acquisition and synthesis on Streptococcus pneumoniae growth and virulence. PLoS ONE 8(1): e49638. doi:10.1371/journal.pone.0049638. 2012 Maqbool, A., Herve, M., Mengin-Lecreulx, D., Wilkinson A.J., and Thomas GH (2012) MpaA is a murein-tripeptide-specific zinc carboxypeptidase that functions as part of a catabolic pathway for peptidoglycan derived peptides in γ-proteobacteria. Biochem. J. 448:329–341. Gruteser N, Marin K, Krämer R, Thomas GH. (2012) Sialic acid utilisation by the soil bacterium Corynebacterium glutamicum. FEMS Microbiol Letts Aug 27. doi: 10.1111/ j.1574-6968.2012.02663.x. Chowdhury N, Norris J, McAlister E, Lau K, Thomas GH, Boyd EF. (2012) The VC1777-VC1779 proteins are members of a sialic acid-specific subfamily of TRAP transporters (SiaPQM) and constitute the sole route of sialic acid uptake in the human pathogen Vibrio cholerae. Microbiology. 158:2158-67 Macdonald, S.J., Lin, G.G, Russell C.W., Thomas, G.H. and Douglas A.E. (2012) The Central Role of the Host Cell in Symbiotic Nitrogen Metabolism Proc Roy Soc B 279:2965-73. Mulligan, C., Leech, A.P., Kelly, D.J. and Thomas, G.H. (2012) The membrane proteins, SiaQ and SiaM, form an essential stoichiometric complex in the sialic acid TRAP transporter SiaPQM (VC1777-1779) from Vibrio cholerae. J. Biol. Chem. 287:3598-608. Clarke, P.A., Mistry, N. and Thomas, G.H. (2012) Synthesis of the Complete Series of Mono Acetates of N-Acetyl-D-Neuraminic Acid Org. Biomol. Chem. 10:529-535. 2011 Thomas, G.H. and Boyd, E.F. (2011) On sialic acid transport and utilisation by Vibrio cholerae. Microbiology. 157:3253 - 3254. MacDonald SJ, Thomas,G.H., Douglas AE (2011) Genetic and metabolic determinants of nutritional phenotype in an insect-bacterial symbiosis. Mol Ecol. 20:2073-84. Maqbool, A., Levdikov, V.M., Blagova, E., Herve, M., Horler, R.S.P., Wilkinson, A.J. and Thomas, G.H. (2011) Compensating stereochemical changes allow murein tripeptide to be accommodated in a conventional peptide binding protein J. Biol. Chem. 286:31512-21 . Mulligan, C., Fischer, M. and Thomas G.H. (2011) Tripartite ATP-independent periplasmic (TRAP) transporters in bacteria and archaea. FEMS Microbiology Reviews 35:68-86. 2010 Fischer, M., Zhang, Q.Y., Hubbard, R.E. and Thomas G.H. (2010) Caught in a TRAP: substrate binding proteins in secondary transport. Trends in Microbiology. 35:68-86. The International Aphid Genomics Consortium (2010) Genome Sequence of the Pea Aphid Acyrthosiphon pisum. PLoS Biol 8(2):e1000313. Wilson ACC, Ashton PD, Calevro F, Charles H, Colella S, Febvay G, Jander G, Kushlan P, Macdonald SA, Schwartz J, Thomas GH and Douglas AE (2010) Genomic insight into the amino acid relations of the pea aphid Acyrthosiphon pisum with its symbiotic bacterium Buchnera aphidicola. Insect Molecular Biology 19 (Suppl. 2), 249–258. Ramsey JS, MacDonald SJ, Jander G, Nakabachi A, Thomas GH and Douglas AE (2010) Genomic evidence for complementary purine metabolism in the pea aphid Acyrthosiphon pisum and its symbiotic bacterium Buchnera aphidicola. Insect Molecular Biology 19 (Suppl. 2), 241–248. Severi, E., Hosie, A.H.F., Hawkhead, J., and Thomas G.H. (2010) Characterisation of a novel sialic acid transporter of the sodium solute symporter (SSS) family and in vivo comparison with known bacterial sialic acid transporters. FEMS Microbiolgy Letters. 304:47-54. 2009 Jackson, A.P., Thomas, G.H., Parkhill, J. and Thomson, N.R. (2009) Evolutionary diversification of an ancient gene family (Rhs) through C-terminal displacement. BMC Genomics10(1):584. Thomas, G.H. (2009) Homes for the orphans: utilization of multiple substrate binding proteins by ABC transporters. Mol Microbiol. 75(1):6-9. Horler, R.S.P., Müller, A., Williamson, D.C., Potts, J.R., Wilson, K.S. and Thomas GH (2009) Furanose-specific sugar transport: characterisation of a bacterial galactofuranose binding protein. J. Biol. Chem. 284(45):31156-63. Basavanna S, Khandavilli S, Yuste J, Cohen JM, Hosie AH, Webb AJ, Thomas GH, Brown JS (2009) Screening of Streptococcus pneumoniae ABC transporter mutants demonstrates that LivJHMGF, a branched-chain amino acid ABC transporter, is necessary for disease pathogenesis. Infect Immun. 77(8):3412-23. Thomas GH, Zucker J, Macdonald SJ, Sorokin A, Goryanin I, Douglas AE (2009) A fragile metabolic network adapted for cooperation in the symbiotic bacterium Buchnera aphidicola. BMC Syst Biol. 3:24. Mulligan C, Geertsma ER, Severi E, Kelly DJ, Poolman B, Thomas GH (2009) The substrate-binding protein imposes directionality on an electrochemical sodium gradient-driven TRAP transporter. Proc Natl Acad Sci U S A. 106(6):1778-83. Md-Saleh SR, Chilvers EC, Kerr KG, Milner SJ, Snelling AM, Weber JP, Thomas GH, Duhme-Klair AK, Routledge A. (2009) Synthesis of citrate-ciprofloxacin conjugates. Bioorg Med Chem Lett. 19(5):1496-8. Horler RS, Butcher A, Papangelopoulos N, Ashton PD, Thomas GH (2009) EchoLOCATION: an in silico analysis of the subcellular locations of Escherichia coli proteins and comparison with experimentally derived locations. Bioinformatics. 25(2):163-6. 2008 Severi, E., Müller, A., Potts, JR., Leech, A., Williamson, D., Wilson, KS and Thomas, G.H. (2008) Sialic acid mutarotation is catalysed by the Escherichia coli beta-propeller protein YjhT. J. Biol. Chem. 283:4841-9. (.pdf) 2007 Severi, E, Hood, DW and Thomas GH. (2007) Sialic acid utilization by bacterial pathogens. Microbiology 153:2817-22. (.pdf) Mulligan, C., Kelly, D.J. and Thomas, G.H. (2007) Tripartite ATP-Independent Periplasmic Transporters: Application of a Relational Database for Genome-Wide Analysis of Transporter Gene Frequency and Organization. J Mol Microbiol Biotechnol. 12:218-26. Chaudhuri R.R. and Thomas G.H. (2007) Understanding the Model and the Menace: a Postgenomic View of Escherichia coli. In Bacterial Pathogenomics. Edited by Mark J. Pallen, M.J., Nelson, K.E. and Preston, G.M. ASM Press May 2007. Thomas GH (2007) Napoleon and typhus: a tale of two generals. Microbiology Today Feb 2007. (.pdf) 2006 Muller, A., Severi, E., Mulligan, C., Watts, A.G., Kelly, D.J., Wilson, K.S., Wilkinson, A.J. and Thomas GH. (2006) Conservation of structure and mechanism in primary and secondary transporters exemplified by SiaP, a sialic acid binding virulence factor from Haemophilus influenzae. J Biol Chem. 281:22212-22. (.pdf) Riley, M., Abe, T., Arnaud, M.B., Berlyn, M.K., Blattner, F.R., Chaudhuri, R.R., Glasner, J.D., Horiuchi, T., Keseler, I.M., Kosuge, T., Mori, H., Perna, N.T., Plunkett, G. 3rd, Rudd, K.E., Serres, M.H. and Thomas, G.H., Thomson, N.R., Wishart, D. and Wanner, B.L. (2006) Escherichia coli K-12: a cooperatively developed annotation snapshot--2005. Nucleic Acids Res. 34:1-9. (.pdf) Prickett, M.D., Page, M., Douglas, A.E. and Thomas GH. (2006) BuchneraBASE: a post-genomic resource for Buchnera sp. APS. Bioinformatics. 22:641-2. (.pdf) Thomas GH, Southworth T, Leon-Kempis MR, Leech A, Kelly DJ. (2006) Novel ligands for the extracellular solute receptors of two bacterial TRAP transporters. Microbiology. 152:187-98. (.pdf) 2005 Severi, E., Randle, G., Kivlin, P., Whitfield, K., Young, R., Moxon, R., Kelly, D., Hood, D. and Thomas, G.H. (2005) Sialic acid transport in Haemophilus influenzae is essential for lipopolysaccharide sialylation and serum resistance and is dependent on a novel tripartite ATP-independent periplasmic transporter. Mol Microbiol. 58:1173-85. (.pdf) Muller, A., Thomas, G.H., Horler, R., Brannigan, J.A., Blagova, E., Levdikov, V.M., Fogg, M.J., Wilson, K.S. and Wilkinson, A.J. (2005) An ATP-binding cassette-type cysteine transporter in Campylobacter jejuni inferred from the structure of an extracytoplasmic solute receptor protein. Mol Microbiol. 57:143-55. (.pdf) Thomas, G.H. (2005) The nicer side of E. coli: Escherichia coli as a model organism. The Biochemist. Dec 2005. (.pdf) Thomas, G.H. (2005) Microbes in the air: John Tyndall and the spontaneous generation debate. Microbiology Today. Nov 2005. (.pdf)70-73 ( |
Meetings & invited presentations2020
2018
2017
2016
2015 Invited speaker at P2P Showcase Meeting, University of Manchester. November 2015. Chair & speaker at CBMNet meeting on "Cell Membrane Stress, Integrity and Engineering", University of Sheffield. Speaker at the Biochemical Society meeting on ABC transporters, University of Chester. Invited speaker at the Industrial Biotechnology Showcase event. London. Chair & speaker at CBMNet meeting on Efflux proteins in Industrial Biotechnology, University of Sheffield. 2014 Invited speaker at University of Warwick, Division of Microbiology and Infection Invited speaker at University of Birmingham, School of Immunity and Infection 2013 Invited speaker at the University of Hull, Centre for Cardiovascular and Metabolic research. Invited speaker at the University of Aberdeen, Institute of Medical Sciences. 2012 Invited keynote speaker at Italian Society of Microbiology & Biotechnology sponsored "Prokaryotes in Cortona" in Tuscany, Italy talking about sialic acid in bacterial pathogens. Invited speaker at Novel Field Based Diagnostics Workshop at FERA talking about substrate binding proteins as biosensors. 2011 Co-organiser of the Biochemical Society's Harden Conference "Metabolic Pathways Analysis 2011" held at the University of Chester in September. Co-organiser of the Society for General Microbiology Spring Meeting Symposium "Insect Symbiosis" held in Harrogate. Download complete abstract book here. Invited speaker at the University of Glasgow, Institute of Infection, Immunity and Inflammation. 2010 Invited speaker at Entomological Society of America Annual Meeting in San Diego. Spoke in Insect Symbiosis symposium. Poster presentation at Sialoglyco 2010 in Potsdam, Germany on sialic acid transport in bacteria. Invited speaker at UK-Japan Systems Biology Meeting at the University of Surrey. Sept. 201. 2009 Invited speaker at StoMP meeting "Noisy Bugs: Modelling and Microbiology" held at the University of Edinburgh in July. Download presentation entitled "Metabolic modelling of a bacterial/animal symbiosis". |